Synaptic Systems - Lamin B1

Cat. No.: HS-404 017

Amount: 200 µl

Price: $415.00

WB

ICC

IHC-P

Background

Lamin B1 (LMNB1) is an intermediate filament-type protein of the nuclear lamina and is ubiquitously expressed throughout development. It plays important roles in many cellular processes like the distribution of heterochromatin and the regulation of gene expression and splicing. The maintenance of LMNB1 protein levels is required for DNA replication and repair and thus mutations in B-type lamins are usually lethal.
Duplication of the LMNB1 gene causes adult-onset autosomal-dominant leukodystrophy (ADLD), a rare neurological disorder in which overexpression of LMNB1 causes progressive central nervous system demyelination. Improper Lamin B1 expression is often present in tumor cells and decreased levels are observed for example in colon cancer, breast cancer and B-cell malignancies. Lamin B1 loss is also a senescence-associated biomarker and distinguishes senescent from proliferating cells in pre-neoplastic lesions or marks senescent cells in various age-related pathologies.

These antibody are suited to distinguish murine from human cells in humanized mouse models.

1 ) Klymenko T et al., Leukemia (2018)
2 ) Yeh CH et al., PLoS ONE (2015)
3 ) Butin-Israeli V et al., Mol. Cell. Biol. (2015)
4 ) Camps J et al., Nucleus (2015)
5 ) Sadaie M et al., Genes Dev. (2013)
6 ) Wazir U et al., Cell. Mol. Biol. Lett. (2013)
7 ) Freund A et al., Mol. Biol. Cell (2012)
8 ) Tang CW et al., J. Cell. Sci. (2008)
9 ) Vergnes L et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
10 ) Moss SF et al., Gut (1999)

Other products for this target

Cat. No. HS-404 017	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: 1 : 100 gallery IHC: not tested yet IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	12G6
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 8 to 19 from human LaminB1 (UniProt Id: P20700)
Reactivity	Reacts with: human (P20700), pig. No signal: mouse (P14733), rat (P70615). Other species not tested yet.
Data sheet	hs-404_017.pdf

Lamin B1 antibody human specific - HS-404 017