Synaptic Systems

Cat. No.: HS-397 017

Amount: 200 µl

Price: $415.00

WB

IHC

IHC-P

IHC-Fr

Background

The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.

1 ) Gordon S et al., BMC Biol. (2017)
2 ) Gordon S et al., Immunol. Rev. (2014)
3 ) Merad M et al., Annu. Rev. Immunol. (2013)
4 ) Lin HH et al., J. Exp. Med. (2005)
5 ) McKnight AJ et al., J. Leukoc. Biol. (1998)

Other products for this target

Cat. No. HS-397 017	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 250 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 100 gallery IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	167B3
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity	Reacts with: mouse (Q61549). No signal: rat. Other species not tested yet.
Remarks	IHC-Fr: PFA and MeOH fixation are recommended.
Data sheet	hs-397_017.pdf

F4/80 antibody - HS-397 017