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F4/80 antibody - HS-397 017

F4/80 is a marker protein for murine macrophages
Rat monoclonal purified IgG
Cat. No.: HS-397 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-397 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 gallery  
Clone 167B3
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Epitop Epitop: AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity Reacts with: mouse (Q61549).
Other species not tested yet.
Specificity Specific for F4/80.
Data sheet hs-397_017.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-397 017
Amount: 200 µl
Price: $415.00
The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.