Tailor-made Antibodies
and Tools for Life Science

F4/80 antibody - HS-397 008

F4/80 is a marker protein for murine macrophages
Rabbit monoclonal recombinant IgG
Cat. No.: HS-397 008
Amount: 100 µl
Price: $420.00
Cat. No. HS-397 008 100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 (see remarks) gallery  
Clone Rb167B3
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity Reacts with: mouse (Q61549).
No signal: rat.
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 167B3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-P: In contrast to the original rat antibody clone 167B3, the rabbit chimeric antibody (cat. no. HS-397 008) shows weak non-specific staining of nucleoli in mouse brain using the SYSY Reference protocol.

Data sheet hs-397_008.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

References for F4/80 - HS-397 008

Nano-modified viruses prime the tumor microenvironment and promote the photodynamic virotherapy in liver cancer.
Ou DL, Liao ZX, Kempson IM, Li L, Yang PC, Tseng SJ
Journal of biomedical science (2024) 311: 1. HS-397 008 IHC-P; tested species: mouse
Genomic and Transcriptomic Landscape of an Oral Squamous Cell Carcinoma Mouse Model for Immunotherapy.
Lee YM, Hsu CL, Chen YH, Ou DL, Hsu C, Tan CT
Cancer immunology research (2023) : . HS-397 008 IHC-P; tested species: mouse
Cat. No.: HS-397 008
Amount: 100 µl
Price: $420.00