Synaptic Systems

Cat. No.: HS-397 004

Amount: 100 µl

Price: $350.00

Zika virus infection causes widespread damage to the inner ear.
Yee KT, Neupane B, Bai F, Vetter DE
Hearing research (2020) 395: 108000. HS-397 004 IHC; tested species: mouse

IHC

IHC-P

Background

The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.

1 ) Gordon S et al., BMC Biol. (2017)
2 ) Gordon S et al., Immunol. Rev. (2014)
3 ) Merad M et al., Annu. Rev. Immunol. (2013)
4 ) Lin HH et al., J. Exp. Med. (2005)
5 ) McKnight AJ et al., J. Leukoc. Biol. (1998)

Other products for this target

Cat. No. HS-397 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery
Immunogen	Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity	Reacts with: mouse (Q61549). Other species not tested yet.
Data sheet	hs-397_004.pdf

F4/80 antibody - HS-397 004

References for F4/80 - HS-397 004