LAMP3 antibody - HS-391 017 K.O.

LAMP3 is a glycoprotein of the lysosomal membrane

Rat monoclonal purified antibody

Cat. No.: HS-391 017

Amount: 100 µg

Price: $415.00

Cat. No. HS-391 017	100 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-354H7
Subtype	IgG2a (κ light chain)
Immunogen	Synthetic peptide corresponding to C-terminal residues of mouse LAMP3. (UniProt Id: Q7TST5)
Reactivity	Reacts with: mouse (Q7TST5), rat (Q5XI99), human (Q9UQV4). Other species not tested yet.
Specificity	Recognizes LAMP 3, no crossreactivity to LAMP 1, LAMP 2 and LAMP 5. K.O. validated
Data sheet	hs-391_017.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-391 017

Amount: 100 µg

Price: $415.00

IHC-P

Background

LAMP 3 (lysosome-associated membrane glycoprotein 3), also referred to as DC-LAMP (dendritic cell LAMP) or CD 208, is a member of the LAMP protein family and a single-pass type I transmembrane protein that can be variably glycosylated. It is specifically expressed in lung type II pneumocytes and by activated human dendritic cells (1, 2).
Recent studies demonstrated high expression of LAMP 3 in a variety of malignancies including squamous cell carcinoma, gastrointestinal cancer, breast cancer, ovarian cancer, and cervical cancer. Its expression has been associated with metastasis and poor overall survival (3, 4, 5).

1 ) de Saint-Vis B et al., Immunity (1998)
2 ) Salaun B et al., Am J Pathol (2004)
3 ) Ozaki K et al., Cancer Res (1998)
4 ) Liao X et al., Int J Mol Sci (2015)
5 ) Wang D et al., Tumour Biol. (2017)

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