Synaptic Systems

Cat. No.: HS-384 017

Amount: 200 µl

Price: $415.00

WB

IHC-P

Background

CD11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells. Antibodies against CD11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).

1 ) Lee JO et al., Cell (1995)
2 ) Lloyd CM et al., J Immunol Methods (2008)
3 ) Duan M et al., Mucosal Immunol (2016)
4 ) Roy A et al., J Biol Chem (2006)

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Cat. No. HS-384 017	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 100 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	194B8D5
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of human CD11b (UniProt Id: P11215)
Reactivity	Reacts with: human (P11215). No signal: mouse (P05555). Other species not tested yet.
Remarks	IHC-P: This antibody shows weak non-specific cross-reactivity to murine macrophages after antigen retrieval at very high temperatures (121°, pressure cooker). Use appropriate controls when optimizing your IHC-P protocol for detection of human cells in humanized mouse models.
Data sheet	hs-384_017.pdf

CD11b antibody human specific - HS-384 017