CD4 antibody mouse specific - HS-360 108

CD4 is a T-cell surface glycoprotein

Rabbit monoclonal recombinant IgG

Cat. No.: HS-360 108

Amount: 100 µl

Price: $415.00

Cat. No. HS-360 108	100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 2000 (AP-staining) gallery IP: not tested yet IHC: 1 : 100 (see remarks) gallery IHC-P: 1 : 900 gallery IHC-Fr: 1 : 100 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb78H9D2
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity	Reacts with: mouse (P06332). No signal: human (P01730), rat (P05540). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone 78H9D2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining. IHC-Fr: PFA and MeOH fixation are possible.
Data sheet	hs-360_108.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

References for CD4 - HS-360 108

Protein Kinase Inhibitor-Mediated Immunoprophylactic and Immunotherapeutic Control of Colon Cancer.
Ghione S, Racoeur C, Mabrouk N, Shan J, Groetz E, Ballot E, Truntzer C, Chouchane L, Végran F, Paul C, Plenchette S, et al.
Frontiers in immunology (2022) 13: 875764. HS-360 108 IHC-P; tested species: mouse

Follicular helper-T cells restore CD8+-dependent antitumor immunity and anti-PD-L1/PD-1 efficacy.
Niogret J, Berger H, Rebe C, Mary R, Ballot E, Truntzer C, Thibaudin M, Derangère V, Hibos C, Hampe L, Rageot D, et al.
Journal for immunotherapy of cancer (2021) 96: . HS-360 108 IHC-P; tested species: mouse

Cat. No.: HS-360 108

Amount: 100 µl

Price: $415.00

IHC

IHC-P

IHC-Fr

Background

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD4 is a co-receptor that assists the T-cell receptor (TCR) in communicating with an antigen-presenting cell.

1 ) Ansari-Lari MA et al., Genome Res. (1996)
2 ) Brady RL et al., Science (1993)
3 ) Prince HE et al., Diagn Clin Immunol (1987)
4 ) Fleischer B et al., J. Immunol. (1986)

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