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Home > Products > 710 006AT647N

mCLING - 710 006AT647N

mCling is a fixable membrane probe for high resolution microscopy
Cat. No.: 710 006AT647N
Amount: 5 nmol
Price: $360.00
Cat. No. 710 006AT647N

5nmol mCLING labeled with ATTO® 647N in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.
Applications
 
ICC: 1 : 50 up to 1 : 250 (1 - 0.2 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (2 - 1 nmol/ml)

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Label ATTO 647N
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.
Data sheet 710_006at647n.pdf

References for mCLING - 710 006AT647N

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).
Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW
eLife (2022) 11: . 710 006AT647N ICC; tested species: human
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface.
Romero-Ramirez A, Casas-Sánchez A, Autheman D, Duffy CW, Brandt C, Clare S, Harcourt K, André MR, de Almeida Castilho Neto KJG, Teixeira MMG, Machado RZ, et al.
PLoS neglected tropical diseases (2022) 169: e0010791. 710 006AT647N ICC
A versatile and customizable low-cost 3D-printed open standard for microscopic imaging.
Diederich B, Lachmann R, Carlstedt S, Marsikova B, Wang H, Uwurukundo X, Mosig AS, Heintzmann R
Nature communications (2020) 111: 5979. 710 006AT647N ICC; tested species: e. coli
Integrative characterization of the near-minimal bacterium Mesoplasma florum.
Matteau D, Lachance JC, Grenier F, Gauthier S, Daubenspeck JM, Dybvig K, Garneau D, Knight TF, Jacques PÉ, Rodrigue S
Molecular systems biology (2020) 1612: e9844. 710 006AT647N ICC
Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis.
Snider CE, Chandra M, McDonald NA, Willet AH, Collier SE, Ohi MD, Jackson LP, Gould KL
Cell reports (2020) 3312: 108526. 710 006AT647N ICC; tested species: fission yeast
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 710 006AT647N ICC; tested species: mouse
Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells.
Zila V, Müller TG, Laketa V, Müller B, Kräusslich HG
mBio (2019) 106: . 710 006AT647N ICC; tested species: human
Reversible supramolecular assembly of velvet worm adhesive fibers via electrostatic interactions of charged phosphoproteins.
Baer A, Hänsch S, Mayer G, Harrington MJ, Schmidt S
Biomacromolecules (2018) : . 710 006AT647N ICC
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA, Lind AL, Smith SE, Li R, Gould KL
eLife (2017) 6: . 710 006AT647N ICC
An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells.
Taniguchi K, Shao Y, Townshend RF, Cortez CL, Harris CE, Meshinchi S, Kalantry S, Fu J, O'Shea KS, Gumucio DL
The Journal of cell biology (2017) 21612: 3981-3990. 710 006AT647N ICC; tested species: human
Mechanoresponsive lipid-protein nanoglobules facilitate reversible fibre formation in velvet worm slime.
Baer A, Schmidt S, Haensch S, Eder M, Mayer G, Harrington MJ
Nature communications (2017) 81: 974. 710 006AT647N ICC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT647N ICC, IHC
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.
Baranov MV, Revelo NH, Dingjan I, Maraspini R, Ter Beest M, Honigmann A, van den Bogaart G
Cell reports (2016) 176: 1518-1531. 710 006AT647N ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT647N ICC, IHC
Ma2/d promotes myonuclear positioning and association with the sarcoplasmic reticulum.
Reuveny A, Shnayder M, Lorber D, Wang S, Volk T
Development (Cambridge, England) (2018) 14517: . 710 006AT647N IHC; tested species: drosophila
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii.
Chartier TF, Deschamps J, Dürichen W, Jékely G, Arendt D
Open biology (2018) 810: . 710 006AT647N IHC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT647N ICC, IHC
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung S, Maritzen T, Wichmann C, Jing Z, Neef A, Revelo NH, Al-Moyed H, Meese S, Wojcik SM, Panou I, Bulut H, et al.
The EMBO journal (2015) 3421: 2686-702. 710 006AT647N IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT647N ICC, IHC
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, et al.
Nature biotechnology (2024) : . 710 006AT647N ExM; tested species: rat
Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and death.
Aber ER, Griffey CJ, Davies T, Li AM, Yang YJ, Croce KR, Goldman JE, Grutzendler J, Canman JC, Yamamoto A
Cell reports (2022) 413: 111480. 710 006AT647N UPTAKE; tested species: mouse
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 710 006AT647N UPTAKE; tested species: mouse
Cat. No.: 710 006AT647N
Amount: 5 nmol
Price: $360.00
Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).
Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW
eLife (2022) 11: . 710 006AT647N ICC; tested species: human
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface.
Romero-Ramirez A, Casas-Sánchez A, Autheman D, Duffy CW, Brandt C, Clare S, Harcourt K, André MR, de Almeida Castilho Neto KJG, Teixeira MMG, Machado RZ, et al.
PLoS neglected tropical diseases (2022) 169: e0010791. 710 006AT647N ICC
A versatile and customizable low-cost 3D-printed open standard for microscopic imaging.
Diederich B, Lachmann R, Carlstedt S, Marsikova B, Wang H, Uwurukundo X, Mosig AS, Heintzmann R
Nature communications (2020) 111: 5979. 710 006AT647N ICC; tested species: e. coli
Integrative characterization of the near-minimal bacterium Mesoplasma florum.
Matteau D, Lachance JC, Grenier F, Gauthier S, Daubenspeck JM, Dybvig K, Garneau D, Knight TF, Jacques PÉ, Rodrigue S
Molecular systems biology (2020) 1612: e9844. 710 006AT647N ICC
Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis.
Snider CE, Chandra M, McDonald NA, Willet AH, Collier SE, Ohi MD, Jackson LP, Gould KL
Cell reports (2020) 3312: 108526. 710 006AT647N ICC; tested species: fission yeast
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 710 006AT647N ICC; tested species: mouse
Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells.
Zila V, Müller TG, Laketa V, Müller B, Kräusslich HG
mBio (2019) 106: . 710 006AT647N ICC; tested species: human
Reversible supramolecular assembly of velvet worm adhesive fibers via electrostatic interactions of charged phosphoproteins.
Baer A, Hänsch S, Mayer G, Harrington MJ, Schmidt S
Biomacromolecules (2018) : . 710 006AT647N ICC
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA, Lind AL, Smith SE, Li R, Gould KL
eLife (2017) 6: . 710 006AT647N ICC
An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells.
Taniguchi K, Shao Y, Townshend RF, Cortez CL, Harris CE, Meshinchi S, Kalantry S, Fu J, O'Shea KS, Gumucio DL
The Journal of cell biology (2017) 21612: 3981-3990. 710 006AT647N ICC; tested species: human
Mechanoresponsive lipid-protein nanoglobules facilitate reversible fibre formation in velvet worm slime.
Baer A, Schmidt S, Haensch S, Eder M, Mayer G, Harrington MJ
Nature communications (2017) 81: 974. 710 006AT647N ICC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT647N ICC, IHC
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.
Baranov MV, Revelo NH, Dingjan I, Maraspini R, Ter Beest M, Honigmann A, van den Bogaart G
Cell reports (2016) 176: 1518-1531. 710 006AT647N ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT647N ICC, IHC
Ma2/d promotes myonuclear positioning and association with the sarcoplasmic reticulum.
Reuveny A, Shnayder M, Lorber D, Wang S, Volk T
Development (Cambridge, England) (2018) 14517: . 710 006AT647N IHC; tested species: drosophila
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii.
Chartier TF, Deschamps J, Dürichen W, Jékely G, Arendt D
Open biology (2018) 810: . 710 006AT647N IHC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT647N ICC, IHC
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung S, Maritzen T, Wichmann C, Jing Z, Neef A, Revelo NH, Al-Moyed H, Meese S, Wojcik SM, Panou I, Bulut H, et al.
The EMBO journal (2015) 3421: 2686-702. 710 006AT647N IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT647N ICC, IHC
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, et al.
Nature biotechnology (2024) : . 710 006AT647N ExM; tested species: rat
Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and death.
Aber ER, Griffey CJ, Davies T, Li AM, Yang YJ, Croce KR, Goldman JE, Grutzendler J, Canman JC, Yamamoto A
Cell reports (2022) 413: 111480. 710 006AT647N UPTAKE; tested species: mouse
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 710 006AT647N UPTAKE; tested species: mouse
ICC
Background
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.
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