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mCLING labeling kit - 710-MCK

mCling is a fixable membrane probe for high resolution microscopy
Cat. No.: 710-MCK
Amount: 50 nmol
Price: $565.00
Cat. No. 710-MCK

Content:
710-MCK A: unlabeled mCling, 50 nmol (lyophilized)
710-MCK B: mCLING purification column
710-MCK C: Washing buffer, 10 ml
710-MCK D: Stop solution, 0.5 ml
710-MCK E: Elution buffer, 1 ml
710-MCK F: Neutralization buffer, 0.5 ml
Storage All components of the kit are stable at 2-8°C.
Shelf life before use: 6 months.
Applications
 
ICC: yes
IHC: yes
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye, but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Only hydrophilic dyes are suitable for effective coupling. Since the dye itself can have an impact on the mCling properties, full functionality of the resulting conjugate cannot be guaranteed.
Data sheet 710-mck.pdf
Documents Instruction manual

References for mCLING labeling kit - 710-MCK

Dense small molecule labeling enables activator-dependent STORM by proximity mapping.
Chen Y, Gu M, Gunning PW, Russell SM
Histochemistry and cell biology (2016) 1463: 255-66. 710-MCK ICC; tested species: human
Cat. No.: 710-MCK
Amount: 50 nmol
Price: $565.00
Dense small molecule labeling enables activator-dependent STORM by proximity mapping.
Chen Y, Gu M, Gunning PW, Russell SM
Histochemistry and cell biology (2016) 1463: 255-66. 710-MCK ICC; tested species: human
Background
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.