|Cat. No. 710-MCK||
All components of the kit are stable at 2-8°C.
Shelf life before use: 6 months.
Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.
Since the dye selected for coupling can have an impact on the mCling properties, full functionality of the resulting conjugate cannot be guaranteed.