mCLING labeling kit - 710-MCK

mCling is a fixable membrane probe for high resolution microscopy

Cat. No.: 710-MCK

Amount: 50 nmol

Price: $565.00

Cat. No. 710-MCK	Content: 710-MCK A: unlabeled mCling, 50 nmol (lyophilized) 710-MCK B: mCLING purification column 710-MCK C: Washing buffer, 10 ml 710-MCK D: Stop solution, 0.5 ml 710-MCK E: Elution buffer, 1 ml 710-MCK F: Neutralization buffer, 0.5 ml
Storage	All components of the kit are stable at 2-8°C. Shelf life before use: 6 months.
Applications	ICC: yes IHC: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Remarks	Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye, but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016. Only hydrophilic dyes are suitable for effective coupling. Since the dye itself can have an impact on the mCling properties, full functionality of the resulting conjugate cannot be guaranteed.
Data sheet	710-mck.pdf
Documents	Instruction manual

References for mCLING labeling kit - 710-MCK

Dense small molecule labeling enables activator-dependent STORM by proximity mapping.
Chen Y, Gu M, Gunning PW, Russell SM
Histochemistry and cell biology (2016) 1463: 255-66. 710-MCK ICC; tested species: human

Cat. No.: 710-MCK

Amount: 50 nmol

Price: $565.00

Background

The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.

1 ) McDonald NA et al., Elife (2017)
2 ) Baranov MV et al., Cell Rep (2016)
3 ) Revelo NH et al., Curr Protoc Neurosci (2016)
4 ) Revelo NH et al., J. Cell Biol. (2014)

Protocols

ICC

IHC