|Cat. No. 710-MCK||
All components of the kit are stable at 2-8°C.
Shelf life before use: 6 months.
Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye, but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.
Only hydrophilic dyes are suitable for effective coupling. Since the dye itself can have an impact on the mCling properties, full functionality of the resulting conjugate cannot be guaranteed.