Synaptic Systems - mCLING labeling kit

Cat. No.: 710-MCK

Amount: 50 nmol

Price: $570.00

Cat. No. 710-MCK	Content: 710-MCK A: unlabeled mCling, 50 nmol (lyophilized) 710-MCK B: mCLING purification column 710-MCK C: Washing buffer, 10 ml 710-MCK D: Stop solution, 0.5 ml 710-MCK E: Elution buffer, 1 ml 710-MCK F: Neutralization buffer, 0.5 ml
Storage	All components of the kit are stable at 2-8°C. Shelf life before use: 6 months.
Applications
Remarks	Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye, but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016. Only hydrophilic dyes are suitable for effective coupling. Since the dye itself can have an impact on the mCling properties, full functionality of the resulting conjugate cannot be guaranteed.
Data sheet	710-mck.pdf
Documents	Instruction manual

Dense small molecule labeling enables activator-dependent STORM by proximity mapping.
Chen Y, Gu M, Gunning PW, Russell SM
Histochemistry and cell biology (2016) 1463: 255-66. 710-MCK ICC; tested species: human

Cat. No.: 710-MCK

Amount: 50 nmol

Price: $570.00

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