pH-sensitive probe fluorescent only under acidic conditions
|Cat. No. 702-rbCpH||
50 µg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS.
Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt!
Avoid bright light to minimize photo bleeching of the fluorescent dye.
|Applications||ICC: yes (see remarks) gallery|
Reacts with: rabbit.
Other species not tested yet.
Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency.
The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.