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anti-rabbit IgG Fab - 702-rbCpH

Polyclonal goat
Cat. No.: 702-rbCpH
Amount: 50 µg
Price: $450.00
Cat. No. 702-rbCpH 50 µg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS.
Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt!
Avoid bright light to minimize photo bleeching of the fluorescent dye.
Applications ICC: yes (see remarks) gallery  
Label CypHer5E
Reactivity Reacts with: rabbit.
Other species not tested yet.
Remarks

Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency.

The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.
ICC: Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark.
Adjust the antibody to the desired dilution and immediately use it for your internalization assay.

Data sheet 702-rbcph.pdf
Cat. No.: 702-rbCpH
Quantity: 50 µg
Price: $450.00