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anti-mouse IgG Fab - 701-msCpH

Polyclonal goat
Cat. No.: 701-msCpH
Amount: 50 µg
Price: $450.00
Cat. No. 701-msCpH 50 µg purified Fab1 fragment, lyophilized, fluorescence-labeled with CypHer5E

CypHer5E is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E can be detected with the filter sets for Cy5TM.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications ICC: yes (see remarks) gallery  
Label CypHer5E
Reactivity Reacts with: mouse.
Other species not tested yet.
Remarks

The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.
ICC: Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark.
Adjust the antibody to the desired dilution and immediately use it for your internalization assay.

Data sheet 701-mscph.pdf
Cat. No.: 701-msCpH
Quantity: 50 µg
Price: $450.00