anti-mouse IgG Fab antibody - 701-msCpH

IMPORTANT NOTE FOR NON-EUROPEAN CUSTOMERS: Please provide us with your import permit for goat-derived biological reagents when ordering this product!

pH-sensitive probe fluorescent only under acidic conditions

Goat polyclonal Fab1 fragment

Cat. No.: 701-msCpH

Amount: 50 µg

Price: $455.00

Cat. No. 701-msCpH	50 µg purified polyclonal Fab1 fragment, lyophilized, fluorescence-labeled with CypHer5E. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	ICC: yes (see remarks) gallery
Label	CypHer5E
Reactivity	Reacts with: mouse. Other species not tested yet.
Remarks	The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment. ICC: Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay.
Data sheet	701-mscph.pdf

Cat. No.: 701-msCpH

Amount: 50 µg

Price: $455.00

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