Tailor-made Antibodies
and Tools for Life Science

von Willebrand factor (vWF) antibody - 527 005

The vWF is a multimeric glycoprotein stored in endothelial cells and thrombocytes
Guinea pig polyclonal purified antibody
Cat. No.: 527 005
Amount: 50 µg
Price: $465.00
Cat. No. 527 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 2000 (see remarks) gallery  
IHC-P: not recommended
IHC-Fr: 1 : 1000 (see remarks) gallery  
IHC-G: 1 : 1000 (see remarks) gallery  
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse von Willebrand factor (UniProt Id: Q8CIZ8)
Reactivity Reacts with: mouse (Q8CIZ8), rat (A0A8J8XVZ5).
Other species not tested yet.
Specificity Does not recognize von Willebrand antigen 2
Remarks

WB: Cat. no. 527 015 is recommended for this application.
ICC: Methanol fixation is recommended.
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining.
IHC-Fr: Acetone, methanol-acetone (1:1 mix) or formaldehyde fixation are recommended.
IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH2O, pH 4.2-4.4, according to Richter et al. 2017 is recommended.

Data sheet 527_005.pdf
Cat. No.: 527 005
Amount: 50 µg
Price: $465.00