Synaptic Systems

Cat. No.: 525 005

Amount: 50 µg

Price: $465.00

WB

ICC

IHC

IHC-P

Background

MLC1 is a membrane protein highly expressed in the distal processes of astrocytes, particularly in perivascular, subpial, and subependymal regions. Recessive mutations in MLC1 genes cause Megalencephalic leukoencephalopathy with subcortical cysts (MLC). Additionally, MLC1 upregulation has been observed in brain tissues affected by multiple sclerosis, Alzheimer’s disease, and Creutzfeldt-Jakob disease.
Although its precise function remains unclear, MLC1 interacts with various proteins, including adhesion proteins such as GlialCAM, chloride channels like CLCN2, and gap junction proteins such as connexin 43. It also modulates receptor tyrosine kinases, including EGFR and Axl, by suppressing their enzymatic activity.
MLC1 plays a crucial role in restoring astrocyte homeostasis after inflammation by inhibiting IL-1β–induced inflammatory signaling pathways (pERK, pNF-kB) (3). Experimental in vitro and ex vivo studies suggest that MLC1 contributes to cell volume regulation in astrocytes during osmotic stress by modulating anion channels (VRAC) and Ca²⁺ influx.
Recent findings indicate that loss of MLC1 disrupts astrocyte–excitatory synapse interactions, potentially affecting extracellular glutamate dynamics under conditions of impaired glutamate transporter activity (5). Furthermore, MLC1 stabilizes membrane structure fluctuations in primary astrocytes by regulating actin filament branching (2).

1 ) Bosch A et al., Front Cell Neurosci (2020)
2 ) Hwang J et al., Mol Brain (2019)
3 ) Brignone MS et al., Mol Neurobiol (2019)
4 ) Morales JE et al., J Neurosci (2022)
5 ) Kater MSJ et al., Glia (2023)

Protocols

Cat. No. 525 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 1000 up to 1 : 2000 (see remarks) gallery IHC-P: 1 : 1000 up to 1 : 2000 gallery
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse MLC1 (UniProt Id: Q8VHK5)
Reactivity	Reacts with: mouse (Q8VHK5 ), rat (D4ABB2). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	525_005.pdf

MLC1 antibody - 525 005