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Home > Products > 522 004

JAM-A antibody - 522 004

JAM-A is a member of the immunoglobulinsuperfamily expressed in tight junctions of epithelial and endothelial cells
Guinea pig polyclonal antiserum
Cat. No.: 522 004
Amount: 100 µl
Price: $370.00
Cat. No. 522 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to extracellular-domain of rat JAM-A (UniProt Id: Q9JHY1)
Reactivity Reacts with: rat (Q9JHY1), mouse (O88792), dog.
Other species not tested yet.
Remarks

ICC: formaldehyde and glyoxal fixation are recommended
Data sheet 522_004.pdf
Cat. No.: 522 004
Amount: 100 µl
Price: $370.00
WB
WB
ICC
ICC
Background

Junctional adhesion molecule-A (JAM-A) is a key transmembrane protein in cell adhesion, immune response, and barrier integrity. It localizes at tight junctions, interacting with ZO-1 and PAR-3 to regulate cell polarity and maintain the blood-brain barrier (BBB) (1). JAM-A also facilitates leukocyte adhesion and transmigration, crucial for immune defense but implicated in inflammatory diseases (1). In the BBB, its downregulation correlates with barrier breakdown in conditions like multiple sclerosis (2). Overexpression in glioblastomas links JAM-A to tumor stem cell maintenance and therapy resistance (3). As a therapeutic target, JAM-A inhibition can reduce leukocyte infiltration and tissue damage, with implications for inflammatory bowel disease, stroke, and cancer (1). Its dual role in junction stabilization and disease progression makes JAM-A a key focus for medical research. JAM-A has a molecular weight of 29 kD which can reach between 32 kD and 43 kD as a result of post-translational modifications (N-glycosylation) or due to differences in mRNA splicing (4).