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Home > Products > 509 005

Prox1 antibody - 509 005

Prox1 is a transcription factor involved in the development of various organs and is a marker for granule cells of the dentate gyrus
Guinea pig polyclonal purified antibody
Cat. No.: 509 005
Amount: 50 µg
Price: $460.00
Cat. No. 509 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 1000 up to 1 : 4000 gallery  
IHC: 1 : 2000 gallery  
IHC-P: 1 : 500 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse Prox1 (UniProt Id: P48437)
Reactivity Reacts with: mouse (P48437), rat (D3ZU00).
Other species not tested yet.
Data sheet 509_005.pdf
Cat. No.: 509 005
Amount: 50 µg
Price: $460.00
ICC
IHC
IHC
IHC
IHC
IHC
IHC-P
Background

The prospero-related homeobox transcription factor 1, Prox1, is important for the embryonic development of various organs (1) including the eye, liver, pancreas, lymphatic system, heart and the central nervous system (CNS) (2). It is a regulator of cell division with impact on e.g. neurogenesis and cancer development and progression. In the CNS, the expression is not restricted to the embryonic development and to precursor cells. Prox1 is a well-known marker for granule cells of the dentate gyrus (DG) during embryonic development, adult neurogenesis but also for mature granule cells in the adult brain (3). Weaker expression can also be detected outside the DG of the adult brain. Prox1 was shown to be expressed in oligodendrocyte progenitors, e.g. in the subventricular zone and the corpus callosum (4) and in a subtype of cortical interneurons (5).