Cat. No.: 508 004
Amount: 100 µl
Price:
$415.00
|
|
|
| Cat. No. 508 004 |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of mouse glutamate dehydrogenase (UniProt Id: P26443) |
| Reactivity |
Reacts with: mouse (P26443), rat (P10860), human (P00367). Other species not tested yet. |
| Specificity | The antibody may also recognize human glutamate dehydrogenase 2 (UniProt Id: P49448) due to sequence homology. |
| Remarks |
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is recommended for immunohistochemical staining. |
| Data sheet | 508_004.pdf |
|
|
Glutamate dehydrogenase, also referred to as GDH or GLUD1, is a mitochondrial enzyme that catalyzes the reversible conversion of L-glutamate to alpha-ketoglutarate and ammonia, thus connecting amino acid metabolism and TCA cycle activity (1,2).
Mammalian glutamate dehydrogenase is composed of six identical subunits, and it is subject to complex allosteric regulation by a wide array of ligands (1,2). While most mammals possess a single GDH enzyme, two isoenzymes encoded by different genes are found in human tissues (1, 2).
Glutamate dehydrogenase is highly expressed in brain, liver, kidney, placenta and pancreas (1). In brain, it is expressed predominantly in astrocytes, responsible for removing and metabolizing the transmitter glutamate (1, 2). In liver and kidney, it functions in ammonia and acid-base homeostasis. In pancreas, it plays a major role in insulin homeostasis (1, 2).
Deregulation of glutamate dehydrogenase is implicated in the pathogenesis of several human disorders ranging from insulin disorders to tumor growth (1, 2).