Glutamate dehydrogenase antibody - 508 004

Glutamate dehydrogenase, also referred to as GDH or GLUD1, is a mitochondrial enzyme that catalyzes the reversible conversion of L-glutamate to alpha-ketoglutarate and ammonia, thus connecting amino acid metabolism and TCA cycle activity (1,2).
Mammalian glutamate dehydrogenase is composed of six identical subunits, and it is subject to complex allosteric regulation by a wide array of ligands (1,2). While most mammals possess a single GDH enzyme, two isoenzymes encoded by different genes are found in human tissues (1, 2).
Glutamate dehydrogenase is highly expressed in brain, liver, kidney, placenta and pancreas (1). In brain, it is expressed predominantly in astrocytes, responsible for removing and metabolizing the transmitter glutamate (1, 2). In liver and kidney, it functions in ammonia and acid-base homeostasis. In pancreas, it plays a major role in insulin homeostasis (1, 2).
Deregulation of glutamate dehydrogenase is implicated in the pathogenesis of several human disorders ranging from insulin disorders to tumor growth (1, 2).

• AP Detection
• ECL Detection
• Fluorescent detection
• Pre-adsorption protocol

• PFA fixation
• Pre-adsorption protocol

• Free floating
• Slide mounted
• Tissue preparation
• Antigen retrieval
• Pre-adsorption protocol

• Chromogenic detection
• Fluorescent detection
• Tissue preparation
• Pre-adsorption protocol