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Home > Products > 506 017

TMEM106B antibody - 506 017 K.O.

TMEM106B forms amyloid-like fibrils in human brain
Rat monoclonal purified IgG
Cat. No.: 506 017
Amount: 100 µg
Price: $420.00
Cat. No. 506 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 1:1000 up to 1 : 1:2000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone SY-118C4
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 239 to 252 from human TMEM106B (UniProt Id: Q9NUM4)
Epitop AA 239 to 252 from human TMEM106B (UniProt Id: Q9NUM4)
Reactivity Reacts with: human (Q9NUM4), mouse (Q80X71).
Other species not tested yet.
Specificity K.O. validated
Remarks

WB: This antibody shows an additional unspecific band at 70kD.
IHC-P: 80% formic acid for 10min and heat mediated antigen retrieval (Tris/EDTA pH 9,0) is recommended for immunohistochemical staining.
Data sheet 506_017.pdf

References for TMEM106B - 506 017

Physiological shedding and C-terminal proteolytic processing of TMEM106B.
Held S, Erck C, Kemppainen S, Bleibaum F, Giridhar NJ, Feederle R, Krenner C, Juopperi SP, Calliari A, Mentrup T, Schröder B, et al.
Cell reports (2024) 441: 115107. 506 017 WB, IHC-P; tested species: human,mouse
Physiological shedding and C-terminal proteolytic processing of TMEM106B.
Held S, Erck C, Kemppainen S, Bleibaum F, Giridhar NJ, Feederle R, Krenner C, Juopperi SP, Calliari A, Mentrup T, Schröder B, et al.
Cell reports (2024) 441: 115107. 506 017 WB, IHC-P; tested species: human,mouse
Cat. No.: 506 017
Amount: 100 µg
Price: $420.00
Physiological shedding and C-terminal proteolytic processing of TMEM106B.
Held S, Erck C, Kemppainen S, Bleibaum F, Giridhar NJ, Feederle R, Krenner C, Juopperi SP, Calliari A, Mentrup T, Schröder B, et al.
Cell reports (2024) 441: 115107. 506 017 WB, IHC-P; tested species: human,mouse
Physiological shedding and C-terminal proteolytic processing of TMEM106B.
Held S, Erck C, Kemppainen S, Bleibaum F, Giridhar NJ, Feederle R, Krenner C, Juopperi SP, Calliari A, Mentrup T, Schröder B, et al.
Cell reports (2024) 441: 115107. 506 017 WB, IHC-P; tested species: human,mouse
WB
IHC-P
Background

TMEM106B or Transmembrane protein 106B is a lysosomal type II transmembrane protein which has been identified by a genome-wide association study as a potential risk factor for a neurodegenerative disorder called frontotemporal lobar degeneration (FTLD)(1). The C-terminal luminal domain of TMEM106B undergoes a proteolytic cleavage that forms sarkosyl-insoluble amyloid-like fibrils (residues 120-254) in human brains (2). TMEM106B is ubiquitously expressed, but the function of TMEM106B is almost unknown. TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry (3).