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Home > Products > 506 017

TMEM106B antibody - 506 017 K.O.

TMEM106B forms amyloid-like fibrils in human brain
Rat monoclonal purified IgG
Cat. No.: 506 017
Amount: 100 µg
Price: $415.00
Cat. No. 506 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 1:1000 up to 1 : 1:2000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone SY-118C4
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 239 to 252 from human TMEM106B (UniProt Id: Q9NUM4)
Epitop AA 239 to 252 from human TMEM106B (UniProt Id: Q9NUM4)
Reactivity Reacts with: human (Q9NUM4), mouse (Q80X71).
Other species not tested yet.
Specificity K.O. validated
Remarks

WB: This antibody shows an additional unspecific band at 70kD.
IHC-P: 80% formic acid for 10min and heat mediated antigen retrieval (Tris/EDTA pH 9,0) is recommended for immunohistochemical staining.
Data sheet 506_017.pdf
Cat. No.: 506 017
Amount: 100 µg
Price: $415.00
WB
IHC-P
Background

TMEM106B or Transmembrane protein 106B is a lysosomal type II transmembrane protein which has been identified by a genome-wide association study as a potential risk factor for a neurodegenerative disorder called frontotemporal lobar degeneration (FTLD)(1). The C-terminal luminal domain of TMEM106B undergoes a proteolytic cleavage that forms sarkosyl-insoluble amyloid-like fibrils (residues 120-254) in human brains (2). TMEM106B is ubiquitously expressed, but the function of TMEM106B is almost unknown. TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry (3).