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Home > Products > 504 005

Tight junction protein ZO-2 antibody - 504 005

ZO-2 plays a role in tight junctions and adherens junctions
Guinea pig polyclonal purified antibody
Cat. No.: 504 005
Amount: 50 µg
Price: $460.00
Cat. No. 504 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
IHC-G: 1 : 500 up to 1 : 1000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse Tight junction protein ZO-2 (UniProt Id: Q9Z0U1)
Reactivity Reacts with: mouse (Q9Z0U1), rat (Q3ZB99), human (Q9UDY2), dog (Q95168).
Other species not tested yet.
Remarks

ICC: Formaldehyde fixation is recommended
IHC-Fr: Acetone fixation is recommended
IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH2O, pH 4.2-4.4, according to Richter et al. 2017 is recommended
Data sheet 504_005.pdf
Cat. No.: 504 005
Amount: 50 µg
Price: $460.00
WB
ICC
IHC-Fr
IHC-G
Background

Tight junction protein ZO-2 is a 160 kDa protein that was first identified by its co-immunoprecipitation with ZO-1 in epithelial cells (1). ZO-2 is encoded by the TJP2 gene, which is located on human chromosome 9 q21.11 (2). ZO-2 is associated with the cytoplasmic surface of tight junctions (TJs), but in non-epithelial cells such as fibroblasts, which do not have TJs, ZO-2 concentrates at adherens junctions (3).
The zona occludens proteins ZO-1, -2 and -3 (also known as TJP1, 2 and 3) are peripheral membrane adaptor proteins that link transmembrane junctional proteins, such as occludin and claudin, to the actin cytoskeleton (4).