Cat. No.: 504 005
Amount: 50 µg
Price:
$460.00
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| Cat. No. 504 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to residues near the carboxy terminus of mouse Tight junction protein ZO-2 (UniProt Id: Q9Z0U1) |
| Reactivity |
Reacts with: mouse (Q9Z0U1), rat (Q3ZB99), human (Q9UDY2), dog (Q95168). Other species not tested yet. |
| Remarks |
ICC: Formaldehyde fixation is recommended |
| Data sheet | 504_005.pdf |
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Tight junction protein ZO-2 is a 160 kDa protein that was first identified by its co-immunoprecipitation with ZO-1 in epithelial cells (1). ZO-2 is encoded by the TJP2 gene, which is located on human chromosome 9 q21.11 (2). ZO-2 is associated with the cytoplasmic surface of tight junctions (TJs), but in non-epithelial cells such as fibroblasts, which do not have TJs, ZO-2 concentrates at adherens junctions (3).
The zona occludens proteins ZO-1, -2 and -3 (also known as TJP1, 2 and 3) are peripheral membrane adaptor proteins that link transmembrane junctional proteins, such as occludin and claudin, to the actin cytoskeleton (4).