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Home > Products > 502 005

PICK1 antibody - 502 005

PICK1 is a scaffolding protein that clusters pre- and postsynaptic neurotransmitter receptors
Guinea pig polyclonal purified antibody
Cat. No.: 502 005
Amount: 50 µg
Price: $460.00
Cat. No. 502 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Full length recombinant mouse PICK1 (UniProt Id: Q62083)
Reactivity Reacts with: mouse (Q62083), rat (Q9EP80).
Other species not tested yet.
Data sheet 502_005.pdf
Cat. No.: 502 005
Amount: 50 µg
Price: $460.00
WB
Background

PICK1 (protein interacts with c-kinase1) is a peripheral membrane protein that regulates the trafficking of pre- and postsynaptic neurotransmitter receptors (1, 2). PICK1 contains a PDZ domain that interacts with the carboxy termini of different receptor proteins (2) and a BAR domain, that mediates the lipid membrane interaction (3).
Via interaction with the endocytic proteins AP2 and Dynamin, PICK1 is involved in the clathrin mediated endocytosis of GluA2 containing AMPA receptors in response to NMDA receptor stimulation (4).