Synaptic Systems

Cat. No.: 498 003

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

NeuroD2, also referred to as Neurogenic differentiation factor 2 or NDRF, is a highly conserved member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. It is highly expressed during development, but it is also present in postmitotic neurons in adulthood, for example in the cerebellum, hippocampus, and cerebral cortex (1).
It is assumed that NeuroD2 acts as a heterodimer with other bHLH transcription factors. It can induce transcription from neuron-specific promoters containing a conserved E-box DNA sequence (1).
NeuroD2 plays a crucial role in the development of the central nervous system (2). It induces neuronal differentiation and promotes neuronal survival (1, 2). Its spectrum of effects includes involvement in the neuronal development in the cerebellum and hippocampus (2, 3), amygdala development and emotional learning (4), and contribution to the terminal neuron localization within the cortical plate (5).
Transgenic mice lacking NeuroD2 do not survive and exhibit severe neurodevelopmental defects (2).

1 ) McCormick MB et al., Mol Cell Biol (1996)
2 ) Olson JM et al., Dev Biol (2001)
3 ) Wilke SA et al., Neural Dev (2012)
4 ) Lin CH et al., Proc Natl Acad Sci U S A (2005)
5 ) Guzelsoy G et al., Sci Rep (2019)

Protocols

ICC

IHC

IHC-P

Cat. No. 498 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 2000 up to 1 : 5000 (see remarks) gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the N-terminal part of mouse NeuroD2 (UniProt Id: Q62414)
Reactivity	Reacts with: mouse (Q62414), rat (Q63689). Other species not tested yet.
Remarks	IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended.
Data sheet	498_003.pdf

NeuroD2 antibody - 498 003