Synaptic Systems - Pyruvate carboxylase

Cat. No.: 494 005

Amount: 50 µg

Price: $465.00

WB

ICC

IHC

Background

Pyruvate carboxylase (PC) is a mitochondrial enzyme catalyzing the ATP-dependent carboxylation of pyruvate to oxaloacetate (1). This is a central biochemical reaction controlling whole-body energetics (2). It is the first step of gluconeogenesis and the main anaplerotic reaction to replenish tricarboxylic acid (TCA) cycle intermediates. Additionally, it is involved in e.g. lipogenesis or insulin secretion.
PC shows high expression in liver and kidney, the primary organs of gluconeogenesis, which synthesize and export glucose into the bloodstream (3).
In the brain, PC plays a critical anaplerotic role during metabolism as well. It is predominately expressed in astrocytes and is needed for the de novo synthesis of glutamate, the main excitatory neurotransmitter (see Featured Topic The Glutamatergic Synapse). Recent literature indicates that PC is also expressed in a subpopulation of neurons (4). There is evidence that gluconeogenesis exists in brain astrocytes (5) and this is becoming more recognized as an important alternative glucose source for neurons, specifically in ischemic stroke and brain tumors. PC has been associated with metabolic reprogramming of cancer cells and tumor progression in a variety of cancer models (2).

1 ) Cesar M et al., J Neurochem (1995)
2 ) Kiesel VA et al., Cancer Metab (2021)
3 ) Sahoo B et al., World J Diabetes (2023)
4 ) Gondáš E et al., Physiol Res (2023)
5 ) Yip J et al., Front Pharmacol (2016)

Protocols

Cat. No. 494 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not tested yet
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Pyruvate carboxylase (UniProt Id: Q05920)
Reactivity	Reacts with: mouse (Q05920), rat (P52873), human (P11498). Other species not tested yet.
Specificity	K.D. validated
Data sheet	494_005.pdf

Pyruvate carboxylase antibody - 494 005 K.D.