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Myosin-11 antibody - 486 003

Myosin-11 is a member of the myosin heavy chain family
Rabbit polyclonal purified antibody
Cat. No.: 486 003
Amount: 50 µg
Price: $375.00
Cat. No. 486 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 4000 (AP-staining) gallery  
ICC: not tested yet
IHC: not tested yet
IHC-P: yes gallery  
IHC-Fr: yes (see remarks) gallery  
Immunogen Recombinant protein corresponding to residues near the carboxy terminus from mouse Myosin-11. (UniProt Id: O08638)
Reactivity Reacts with: human (P35749), mouse (O08638), rat (Q63862).
Other species not tested yet.
Specificity Specific for Myosin-11, detect only isoform1.
Remarks

IHC-Fr: methanol fixation is recommended

Data sheet 486_003.pdf
Cat. No.: 486 003
Amount: 50 µg
Price: $375.00
Background

Myosin-11 also called smooth muscle myosin heavy chain or SMMHC is a myosin expressed in smooth muscle that functions as a primary contractile protein. Myosin-11 is a common myoepithelial marker in immunohistochemistry, showing cytoplasmic and membranous positivity in myoepithelial and smooth muscle cells. It tends to provide more sensitive results with less myofibroblast cross-reactivity when compared to biomarkers like calponin and smooth muscle actin (1). Four Myosin-11 isoforms exist as a result of alternative splicing of the mRNA product of a single gene (2- 5).