Synaptic Systems

Cat. No.: 483 005

Amount: 50 µg

Price: $465.00

A single-cell transcriptomic map of the developing Atoh1 lineage identifies neural fate decisions and neuronal diversity in the hindbrain.
Butts JC, Wu SR, Durham MA, Dhindsa RS, Revelli JP, Ljungberg MC, Saulnier O, McLaren ME, Taylor MD, Zoghbi HY
Developmental cell (2024) : . 483 005 IHC; tested species: mouse

ICC

IHC

Background

Tbr2 also known as Eomesodermin is a T-domain transcription factor playing a crucial role during brain development (1). In the developing neocortex Tbr2 is upregulated during the transition from radial glia to to intermediate progenitor cells (2). Tbr2 disruption has shown its involvement in trophoblast differentiation and later in gastrulation (3). Furthermore, it functions in the differentiation of CD8+ T-cells during immune response (4).

1 ) Bulfone A et al., Mech Dev (1999)
2 ) Englund C et al., J. Neurosci. (2005)
3 ) Russ AP et al., Nature (2000)
4 ) Pearce EL et al., Science (2003)

Protocols

ICC

IHC

Cat. No. 483 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Tbr2 (UniProt Id: O54839)
Reactivity	Reacts with: mouse (O54839), rat (D3ZY52). Other species not tested yet.
Data sheet	483_005.pdf

Tbr2 antibody - 483 005

References for Tbr2 - 483 005