|Cat. No. 482 005||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 (see remarks) gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not recommended
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 200 gallery
Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery
|Immunogen||Recombinant protein corresponding to residues near the carboxy terminus of mouse Claudin5. (UniProt Id: O54942)|
Reacts with: rat (Q9JKD6), mouse (O54942), dog.
Other species not tested yet.
ICC: Methanol fixation is recommended
Claudin5 is predominantly expressed in endothelial cells, especially brain endothelial cells (1) and therefore it is thought that the paracellular permeability of the blood–brain barrier is largely determined by the expression levels of Claudin5. An initial study using Claudin5 knockout (KO) mice clearly showed that expression of Claudin5 in the blood–brain barrier is essential for preventing the entrance of molecules with molecular weights between 400 and 800 Da in the brain. (2) The size-selective modulation of blood–brain barrier permeability has an advantage in comparison to clinically performed methods for increasing blood–brain barrier permeability, namely intra-carotid hyperosmolar mannitol administration. Mannitol administration completely disrupts the blood–brain barrier by withdrawing water from endothelial cells and enabling the entrance of bloodborne proteins into the brain. (3,4)