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Neuron-glial antigen 2 antibody - 481 003

The NG2 is a valuable marker for several incompletely differentiated precursor cells.
Rabbit polyclonal purified antibody
Cat. No.: 481 003
Amount: 50 µg
Price: $450.00
Cat. No. 481 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: not recommended
IHC: not tested yet
IHC-P: not recommended
IHC-Fr: 1 : 200 up to 1 : 500 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse Neuron- glial antigen 2 (UniProt Id: Q8VHY0)
Reactivity Reacts with: mouse (Q8VHY0), rat (Q00657).
Other species not tested yet.
Specificity Specific for Neuron-glial antigen 2
Remarks

IHC-Fr: methanol fixation is recommended

Data sheet 481_003.pdf
Cat. No.: 481 003
Amount: 50 µg
Price: $450.00
Background

The NG2 proteoglycan is a type I membrane protein that is expressed by a variety of immature cells of several embryonic tissue origins including glia, muscle progenitor cells, and pericytes (1). In the central nervous system, expression of NG2 was originally thought to specify oligodendroglial progenitor cells, but more recent data suggest that NG2-expressing cells encompass a wider range of immature glial cells in white and gray matter. These include glia that make synaptic-like contacts with neurons in the hippocampus and cerebellum (2) and glial cells specifically associated with the nodes of Ranvier (3). Interestingly, many NG2-positive cells are both proliferative and motile or exhibit local process motility (4, 5).