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P2Y12 antibody - 476 008

P2Y12 is a Gi - coupled purinergic receptor relevant for microglia activation and platelet aggregation.
Rabbit monoclonal recombinant IgG
Cat. No.: 476 008
Amount: 50 µg
Price: $415.00
Cat. No. 476 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 10000 gallery  
IHC-P: 1 : 1000 up to 1 : 10000 gallery  
Clone Rb341D12
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse P2Y12 receptor (UniProt Id: Q9CPV9)
Reactivity Reacts with: mouse (Q9CPV9).
No signal: rat (Q9EPX4).
Other species not tested yet.

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 341D12. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 476_008.pdf
Cat. No.: 476 008
Amount: 50 µg
Price: $415.00

P2Y12 receptor (P2RY12) is a Gi – coupled purinoceptor and is of particular relevance for microglia in the central nervous system (CNS) (1). The preferred agonist is ADP, the degradation product of ATP, which is released from neurons and other glial cells during physiological activity or after tissue damage. P2Y12 receptor is highly expressed in processes and somata of surveilling microglia and plays a major role in microglial chemotaxis in response to local CNS injury (2). More recently, P2Y12 receptors have been shown to be concentrated at microglia process-neuronal somata contacts (3) and to be critical for neuroprotection. To date, P2Y12 receptor is one of the most accepted microglia-specific markers used to distinguish CNS-resident microglia from CNS-associated macrophages (CAMs) and infiltrating monocytes/macrophages (4). The expression level of P2Y12 receptor is downregulated in an activated state of microglia referred to as disease-associated microglia (DAM). In the periphery, P2Y12 receptor is expressed in platelets and is a well-known biological target for anti-thrombotic drugs due to its central role in platelet activation, aggregation and blood clotting (5).