|Cat. No. 475 003||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 1000 gallery
|Immunogen||Full length mouse recombinant CaBP 5 (UniProt Id: D3ZW89)|
Reacts with: mouse (Q9JLK3), rat (D3ZW89).
Other species not tested yet.
|Specificity||Due to sequence homology, the antibody may exhibit some cross-reactivity to CaBP1, 2, 3 and 4.|
IHC: Brief immersion fixation of retina samples gives best results. For over night fixed tissues, a heat mediated antigen retrieval (AGR) can be applied.
Calcium binding protein 5 (CaBP 5) belongs to a subfamiliy of neuronal Ca2+ binding proteins (CaBP 1-5) that show a high similarity to Calmodulin (1).
It is expressed in rod and cone bipolar cells of the retina (1) and cochlea inner hair cells of the ear (2).
CaBPs are involved in the modulation of voltage gated calcium channels, TRP channels and inositol1,4,5-triphosphate (IP3) receptors (2).
A CaBP 5 K.O. model suggests that this Ca-binding protein is involved in the rod-mediated response across the retina (3).