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P-glycoprotein 1 antibody - 473 005

P-glycoprotein1 is one of the most important efflux transporters.
Guinea pig polyclonal purified antibody
Cat. No.: 473 005
Amount: 50 µg
Price: $450.00
Cat. No. 473 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP-staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not recommended
IHC-P: not tested yet
IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse P-glycoprotein 1. (UniProt Id: P06795)
Reactivity Reacts with: mouse (P06795), rat (P43245).
Other species not tested yet.
Specificity Specific for P-glycoprotein 1
Remarks

WB: P-glycoprotein1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-Fr: PFA-fixation is recommended

Data sheet 473_005.pdf
Cat. No.: 473 005
Amount: 50 µg
Price: $450.00
Background

P-glycoprotein (Pgp) is a member of the ATP-binding cassette (ABC) superfamily of transport proteins that utilize ATP to translocate a variety of lipophilic substrates across biological membranes. (1) Pgp is encoded for by a family of genes referred to as the multidrug resistance or MDR genes [2, 3] whose products function as multidrug efflux pumps [3, 4].
P-glycoprotein (Pgp) is one of the proteins expressed naturally on the plasmatic membranes of endothelial cells at the blood-brain barrier (BBB). It protects the brain from harmful substances by excluding them from entering into the parenchyma from blood circulation. It is one of several so-called efflux pumps present at the BBB and in other organs. Overexpression of this protein excludes a wide range of therapeutics for use as treatment for Central Nervous System (CNS) disorders. (5)