Synaptic Systems

Cat. No.: 471 005

Amount: 50 µg

Price: $465.00

Dot blot

IHC

IHC-P

Background

Glucagon-like peptide-1 (GLP-1) is a member of the glucagon family of hormones.
The well conserved peptide is generated by tissue-specific posttranslational processing of the proglucagon precursor, yielding the amidated GLP-1(7-36)amide or the non-amidated GLP-1(7-37) (1). In humans and mice, GLP-1 is predominantly amidated, whereas in rats both amidated and nonamidated GLP-1 is present (1,2). Both forms are biologically active and rapidly inactivated by proteolytic cleavage (1,3).
GLP-1 is produced and secreted by intestinal enteroendocrine L-cells and certain neurons within the nucleus of the solitary tract in the brainstem in response to nutrient ingestion (1). Although there is some species variation, the density of L-cells increases distally along the gut, with greatest density in the ileum and colon (1,2).
GLP-1 exerts its effects via interaction with its specific G-protein-coupled receptor GLP-1R. Receptor binding stimulates insulin secretion from pancreatic beta cells.
In addition to its insulinotropic effects, GLP-1 promotes beta cell proliferation, it is involved in gastrointestinal motility, and it inhibits gastric emptying. In bone, GLP-1 inhibits bone resorption. In the brain, GLP-1 is thought to be involved in memory formation as well as in the control of appetite (1,3).
The numerous beneficial effects of GLP-1 render this hormone an interesting candidate for the development of pharmacotherapies to treat obesity and diabetes (4,5).

1 ) Müller TD et al., Mol Metab (2019)
2 ) Kuhre RE et al., Peptides (2014)
3 ) Seino Y et al., J Diabetes Investig (2010)
4 ) Sekar R et al., Int Rev Cell Mol Biol (2016)
5 ) He J et al., Drug Discov Today (2020)

Protocols

Cat. No. 471 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet Dot blot: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 1000 up to 1 : 2000 gallery
Immunogen	Synthetic peptide comprising almost all of the processed mouse GLP-1(7-36)amide. (UniProt Id: P55095)
Reactivity	Reacts with: mouse (P55095), rat (P06883). Other species not tested yet.
Specificity	The antibody recognizes GLP-1 with a preference for the amidated GLP-1(7-36)amide. It may show some cross-reactivity with the unprocessed precursor protein.
Remarks	Dot blot: This application was tested with synthetic peptides only.
Data sheet	471_005.pdf

GLP-1 antibody - 471 005