Synaptic Systems

Cat. No.: 468 003

Amount: 50 µg

Price: $375.00

IHC

IHC-P

Background

Ghrelin is an orexigenic peptide hormone belonging to the motilin family. It is a well conserved 28 amino acid peptide generated by post-translational cleavage of the preproghrelin precursor protein (1).
Ghrelin is produced predominantly by endocrine X/A-like cells of the stomach submucosa, from where it is secreted into the plasma (1). It is also present in other parts of the gastrointestinal tract, while substantially lower amounts derive from other tissues including the pancreas. In pancreatic islets, ghrelin producing epsilon cells are primarily found during gestational development (2,3). After birth, epsilon cell numbers gradually decline (2,3).
Ghrelin circulates in two major forms: acyl-ghrelin, which possesses an n-octanoyl modification at Ser3, and des-acyl ghrelin without this modification (1). Des-acyl ghrelin is the predominant circulating form of ghrelin, although the lipid modification is required for binding to the growth hormone secretagogue receptor GHSR, which induces growth hormone release from the pituitary gland (1,3).
Ghrelin has an appetite stimulating effect, induces adiposity, and it regulates gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It also plays a crucial role in cardioprotection, muscle atrophy, bone metabolism and cancer (3,4).
Inhibitors of ghrelin have attracted enormous interest as potential anti-obesity therapeutic targets (5).

1 ) Kojima M et al., Nature (1999)
2 ) Walia P et al., Pediatr Res (2009)
3 ) Chanclón B et al., Front Endocrinol (Lausanne) (2012)
4 ) Pradhan G et al., Curr Opin Clin Nutr Metab Care (2013)
5 ) Foster-Schubert KE et al., Endocr Rev (2006)

Protocols

IHC

IHC-P

Cat. No. 468 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic octanoylated Ghrelin peptide corresponding to AA 24 to 51 from mouse Ghrelin precursor (UniProt Id: Q9EQX0). (UniProt Id: Q9EQX0)
Reactivity	Reacts with: mouse (Q9EQX0), rat (Q9QYH7). Other species not tested yet.
Specificity	The antibody is specific for Ghrelin. It may crossreact with the unprocessed precursor protein.
Data sheet	468_003.pdf

Ghrelin antibody - 468 003