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Home > Products > 464 004

Aldolase C antibody - 464 004

Aldolase C a brain type isozyme of a glycolysis enzyme
Guinea pig polyclonal antiserum
Cat. No.: 464 004
Amount: 100 µl
Price: $365.00
Cat. No. 464 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 364 from human ALDOC (UniProt Id: P09972)
Reactivity Reacts with: mouse (P05063), rat (P09117).
Other species not tested yet.
Specificity Specific for Aldolase C, no cross-reactivity to Aldolase A and B.
Remarks

ICC: Methanol fixation is recommended.
Data sheet 464_004.pdf

References for Aldolase C - 464 004

Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity.
Voerman S, Urbanus BHA, Schonewille M, White JJ, De Zeeuw CI
Communications biology (2022) 51: 1328. 464 004 IHC; tested species: mouse
Cat. No.: 464 004
Amount: 100 µl
Price: $365.00
Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity.
Voerman S, Urbanus BHA, Schonewille M, White JJ, De Zeeuw CI
Communications biology (2022) 51: 1328. 464 004 IHC; tested species: mouse
WB
WB
ICC
ICC
IHC
IHC
IHC
IHC
IHC
IHC
Background

Fructose 1, 6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1, 6-bisphosphate. Fructose 1, 6-bisphosphate aldolase exists as three forms, the muscle-specific Aldolase A, the liver-specific aldolase B, and the brain-specific aldolase C. (1)
Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells (2). Aldolase C is also expressed in astrocytes (3)