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Home > Products > 463 005

ENT4 antibody - 463 005

ENT4 is a plasma membrane monoamine transporter
Guinea pig polyclonal purified antibody
Cat. No.: 463 005
Amount: 50 µg
Price: $465.00
Cat. No. 463 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 505 to 528 from mouse Equilibrative nucleoside transporter 4 (UniProt Id: Q8R139)
Reactivity Reacts with: mouse (Q8R139), rat (F1M2Y4).
Other species not tested yet.
Data sheet 463_005.pdf
Cat. No.: 463 005
Amount: 50 µg
Price: $465.00
WB
ICC
IHC
IHC
IHC
Background

ENT4 (equilibrative nucleoside transporter 4), also known as SLC29A4 (solute carrier family 29 member 4) or PMAT, is a 530 amino acid plasma membrane protein residues with 10–12 transmembrane segments (1). This protein catalyzes the reuptake of monoamines into presynaptic neurons, thus determining the intensity and duration of monoamine neural signaling (4).
ENT4 was located to the endothelial cells in the Blood brain barrier (2, 5), to the luminal membrane of choroid epithelial cells (3), and to neurons (1, 4).