Tailor-made Antibodies
and Tools for Life Science

Collagen type IV antibody - 462 004

Collagen type IV is a basement membrane protein
Guinea pig polyclonal antiserum
Cat. No.: 462 004
Amount: 100 µl
Price: $370.00
Cat. No. 462 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: yes
ICC: not tested yet
IHC: 1 : 250 up to 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 100 (see remarks) gallery  
IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-G: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Immunogen human heterotrimeric Collagen type IV containing two α 1-like (UniProt Id: P02462) and one α 2-like (UniProt Id: P08572) chains.
Reactivity Reacts with: mouse (P02463), rat (F1MA59), human (P02462), human (P08572), mouse (P08122), rat (F1M6Q3).
Other species not tested yet.
Remarks

IHC: antigen retrieval (0.2 mg/ml pepsin in 0.2 M HCl for 20 min at 37 °C) is recommended.
IHC-P: strong antigen retrieval is necessary
-  e.g.: antigen retrieval with Tris- EDTA buffer pH 9.0 in a food steamer followed with pepsin antigen retrieval or an antigen retrieval in a pressure cooker
IHC-Fr: PFA fixation is recommended.
IHC-G: Fixation with 3% glyoxal, 1% acetic acid, 20% ethanol in ddH2O according to Richter et al. 2017, or 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 are recommended.

Data sheet 462_004.pdf
Cat. No.: 462 004
Amount: 100 µl
Price: $370.00