|Cat. No. 456 004||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 1000 up to 1 : 2000 gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 655 to 674 from rat Glutaminase (UniProt Id: P13264)|
Reacts with: mouse, rat (P13264).
Other species not tested yet.
|Specificity||Specific for Glutaminase 1|
|Matching control protein/peptide||456-0P|
Glutamate is the major excitatory neurotransmitter in the central nervous system. During neurotransmission it is released from synaptic vesicles into the synaptic cleft and is sensed by post-synaptic glutamate receptors (GluAs) (1).
Excitatory amino acid transporters (EAATs) translocate extracellular glutamate into neighbouring astrocytes where it is converted to glutamine by glutamine synthetase (2, 3, 4).
The glutamine is shuttled back to neurons where it is processed to glutamate by glutaminases (GLS) (5). This postulated Glu/Gln cycle is a key mechanism for the homeostatic control of these amino acids.
In humans, three isoforms (GLS1, 2 and 3) have been described. GLS1 is highly expressed in brain and kidney whereas GLS2 is mainly expressed in cardiac and skeletal muscle. GLS3 shows a broader tissue distribution (5). The GLS1 precursor is processed to a 65 and 68 kDa chain.