|Cat. No. 453 004||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 250 up to 1 : 500 gallery
|Immunogen||Synthetic peptide corresponding to AA 489 to 505 from mouse Neurocan (UniProt Id: P55066)|
Reacts with: rat (P55067), mouse (P55066).
Other species not tested yet.
|Specificity||Specific for full length Neurocan and the N-terminal fragment (CSPG-190). Does not detect the c-terminal fragment (CSPG-150).|
Neurocan is a secreted brain-specific chondroitin sulfate proteoglycan (CSPG) whose expression and proteolytic cleavage are developmentally regulated (1). It belongs to the lectican family, is a component of the extracellular matrix and interacts with numerous molecules like Hyalorunan, Tenascins, N-CAM and more (2).
It is processed into a C-terminal (CSPG-150) and an N-terminal (CSPG-190) core glycoprotein which are the major forms occuring in adult rodent brain.
In young brains unprocessed Neurocan can also be detected together with both proteolytic products while its expression drops during development and is hardly detectable In adult brains (3).