Synaptic Systems

Cat. No.: 452 005

Amount: 50 µg

Price: $465.00

Cat. No. 452 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 1000 up to 1 : 2000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 134 to 162 from mouse Pro-opiomelanocortin (UniProt Id: P01193)
Reactivity	Reacts with: mouse (P01193), rat (P01194). Other species not tested yet.
Specificity	The antibody is specific for ACTH. It may show minor cross-reactivity to the unprocessed precursor protein.
Remarks	IHC-P: For chromogenic detection, an optimized AGR time of 10 minutes is recommended for best results. For best results apply mild antigen retrieval.
Data sheet	Datasheet 452_005

References for ACTH - 452 005

Dominant Expression of Chromogranin B in Pituitary Corticotrophs and Its Putative Role in Interaction With Secretogranin III.
Kikuchi S, Odashima K, Yasui T, Torii S, Hosaka M, Gomi H
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2025) 731-2: 29-53. 452 005 IHC, EM; tested species: mouse,rat

Cat. No.: 452 005

Amount: 50 µg

Price: $465.00

IHC

IHC-P

Background

Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a 39 amino acid peptide hormone. It is synthesized from the precursor POMC (pro-opiomelanocortin). POMC is proteolytically processed in a cell type specific manner to generate a couple of small biologically active peptides including ACTH (1,2).
ACTH is mainly produced and secreted by corticotroph cells of the anterior pituitary in response to corticotropin-releasing hormone from the hypothalamus. ACTH acts on the adrenal cortex of the adrenal gland to stimulate the production of glucocorticoids like cortisol. ACTH plays a pivotal role in homeostasis and stress response and is a key component of the hypothalamic-pituitary-adrenal (HPA) axis (2,3).
ACTH is also produced in extra-pituitary sites like hypothalamus and skin, where further processing of ACTH leads to the generation of alpha melanocyte-stimulating hormone (α-MSH) (2).
An excess of ACTH can cause Cushing's syndrome whereas ACTH deficiency can result in secondary adrenal insufficiency (3,4).

1 ) Imura H et al., Clin Endocrinol Metab (1985)
2 ) Stevens A et al., Results Probl Cell Differ (2010)
3 ) Gallo-Payet N et al., J Mol Endocrinol (2016)
4 ) Novoselova TV et al., Endocr Connect (2019)

Protocols

IHC

IHC-P

ACTH antibody - 452 005

References for ACTH - 452 005