|Cat. No. 452 005||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 1000 up to 1 : 2000 (see remarks) gallery
|Immunogen||Synthetic peptide corresponding to AA 134 to 162 from mouse Pro-opiomelanocortin (UniProt Id: P01193)|
Reacts with: mouse (P01193), rat (P01194).
Other species not tested yet.
|Specificity||The antibody is specific for ACTH. It may show minor cross-reactivity to the unprocessed precursor protein.|
IHC-P: For best results apply short antigen retrieval (10 min, citrate pH 6.0 at 97°C).
Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a 39 amino acid peptide hormone. It is synthesized from the precursor POMC (pro-opiomelanocortin). POMC is proteolytically processed in a cell type specific manner to generate a couple of small biologically active peptides including ACTH (1,2).
ACTH is mainly produced and secreted by corticotroph cells of the anterior pituitary in response to corticotropin-releasing hormone from the hypothalamus. ACTH acts on the adrenal cortex of the adrenal gland to stimulate the production of glucocorticoids like cortisol. ACTH plays a pivotal role in homeostasis and stress response and is a key component of the hypothalamic-pituitary-adrenal (HPA) axis (2,3).
ACTH is also produced in extra-pituitary sites like hypothalamus and skin, where further processing of ACTH leads to the generation of alpha melanocyte-stimulating hormone (α-MSH) (2).
An excess of ACTH can cause Cushing's syndrome whereas ACTH deficiency can result in secondary adrenal insufficiency (3,4).