Cat. No.: 452 005
Amount: 50 µg
Price:
$460.00
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| Cat. No. 452 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to AA 134 to 162 from mouse Pro-opiomelanocortin (UniProt Id: P01193) |
| Reactivity |
Reacts with: mouse (P01193), rat (P01194). Other species not tested yet. |
| Specificity | The antibody is specific for ACTH. It may show minor cross-reactivity to the unprocessed precursor protein. |
| Remarks |
IHC-P: For best results apply short antigen retrieval (10 min, citrate pH 6.0 at 97°C). |
| Data sheet | 452_005.pdf |
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Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a 39 amino acid peptide hormone. It is synthesized from the precursor POMC (pro-opiomelanocortin). POMC is proteolytically processed in a cell type specific manner to generate a couple of small biologically active peptides including ACTH (1,2).
ACTH is mainly produced and secreted by corticotroph cells of the anterior pituitary in response to corticotropin-releasing hormone from the hypothalamus. ACTH acts on the adrenal cortex of the adrenal gland to stimulate the production of glucocorticoids like cortisol. ACTH plays a pivotal role in homeostasis and stress response and is a key component of the hypothalamic-pituitary-adrenal (HPA) axis (2,3).
ACTH is also produced in extra-pituitary sites like hypothalamus and skin, where further processing of ACTH leads to the generation of alpha melanocyte-stimulating hormone (α-MSH) (2).
An excess of ACTH can cause Cushing's syndrome whereas ACTH deficiency can result in secondary adrenal insufficiency (3,4).