Cat. No.: 448 003
Amount: 50 µg
|Cat. No. 448 003||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet
|Immunogen||Recombinant protein corresponding to AA 629 to 719 from mouse Elfn1 (UniProt Id: Q8C8T7)|
Reacts with: mouse (Q8C8T7), rat (D3ZZ44).
Other species not tested yet.
|Specificity||Specific for ELFN 1 K.O. validated PubMed: 35221979|
Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1 (ELFN1) is a a postsynaptic protein that regulates circuit dynamics in the central nervous system (1) and is expressed with increasing levels during postnatal development (2).
ELFN1 is involved in the generation of facilitating synapses onto somatostatin interneurons by the recruitment of the two pre-synaptic components mGluR7 and GluK2 (2, 3).
Missense mutations of ELFN1 in the carboxy-terminal region required for mGluR7 recruitment have been observed in patients with epilepsy and attention deficit hyperactivity disorder (ADHD) (2).