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Home > Products > 446 004

Galanin antibody - 446 004

Galanin is a neuropeptide with impact on diverse physiological functions including arousal/sleep, nociception, feeding and cognition
Guinea pig polyclonal antiserum
Cat. No.: 446 004
Amount: 100 µl
Price: $370.00
Cat. No. 446 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 up to 1 : 2500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 33 to 61 from mouse Galanin (UniProt Id: P47212)
Reactivity Reacts with: mouse (P47212), rat (P10683).
Other species not tested yet.
Specificity The antibody is specific for galanin. It may crossreact with the unprocessed precursor protein and with galanin-like peptide due to sequence homology.
Data sheet 446_004.pdf
Cat. No.: 446 004
Amount: 100 µl
Price: $370.00
ICC
IHC
IHC
IHC
IHC-P
IHC-P
IHC-P
Background

Galanin, also referred to as Gal or Galn, is a 30 amino acid neuropeptide (29 in rodents) which was first isolated from porcine intestine in 1983 (1). It is synthesized as part of a larger propeptide which is proteolytically processed to release the mature neuropeptide. Galanin is highly conserved across species and has a widespread presence in the periphery and the central nervous system, including brain, spinal cord, and gastrointestinal tract. The effects of this neuropeptide are mediated through three G protein coupled galanin receptors (2, 3).
Galanin has been implicated in many biologically diverse functions including arousal/sleep regulation, nociception, cognition, feeding, reproduction, osmotic homeostasis, glucose metabolism, and regulation of mood (3, 4).
Several human diseases, including Alzheimer’s disease, epilepsy, diabetes mellitus and mood disorders like anxiety and depression are associated with the disturbance of the galaninergic system (2, 3).