Synaptic Systems

Cat. No.: 445 003

Amount: 50 µg

Price: $385.00

Cat. No. 445 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery IP: not tested yet ICC: 1 : 1000 gallery IHC: 1 : 1000 (see remarks) gallery IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 633 from mouse BCAS1 (UniProt Id: Q80YN3)
Reactivity	Reacts with: mouse (Q80YN3), rat (Q3ZB98-1), human (O75363). Other species not tested yet.
Specificity	Specific for BCAS1 and its known isoforms
Remarks	IHC: AGR can improve signal strength and reveals additional immunoreactive structures.
Data sheet	445_003.pdf

References for BCAS1 - 445 003

Oligodendrocytes produce amyloid-β and contribute to plaque formation alongside neurons in Alzheimer's disease model mice.
Sasmita AO, Depp C, Nazarenko T, Sun T, Siems SB, Ong EC, Nkeh YB, Böhler C, Yu X, Bues B, Evangelista L, et al.
Nature neuroscience (2024) : . 445 003 IHC; tested species: mouse

Cerebral Creatine Deficiency Affects the Timing of Oligodendrocyte Myelination.
Rosko LM, Gentile T, Smith VN, Manavi Z, Melchor GS, Hu J, Shults NV, Albanese C, Lee Y, Rodriguez O, Huang JK, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 437: 1143-1153. 445 003 IHC; tested species: mouse

Oligodendrocyte death initiates synchronous remyelination to restore cortical myelin patterns in mice.
Chapman TW, Olveda GE, Bame X, Pereira E, Hill RA
Nature neuroscience (2023) 264: 555-569. 445 003 IHC; tested species: mouse

PBAF Subunit Pbrm1 Selectively Influences the Transition from Progenitors to Pre-Myelinating Cells during Oligodendrocyte Development.
Waldhauser V, Baroti T, Fröb F, Wegner M
Cells (2023) 1212: . 445 003 IHC; tested species: mouse

Cat. No.: 445 003

Amount: 50 µg

Price: $385.00

ICC

IHC

IHC-P

Background

Breast carcinoma-amplified sequence 1 (BCAS1) was originally identified as a potential oncogene amplified in human cancer cell lines (1). It is also highly expressed in brain (2) where it mainly defines an oligodendroglial subpopulation occuring at regions of active myelin formation (3).
In fetal brain BCAS1 positive oligodendrocytes are restricted to the white matter. In adult brains they persist in the grey matter until old age (3).

1 ) Collins C et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
2 ) Ishimoto T et al., Glia (2017)
3 ) Fard MK et al., Sci Transl Med (2017)

Protocols

ICC

IHC

IHC-P

Other products for this target

BCAS1 antibody - 445 003

References for BCAS1 - 445 003