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Home > Products > 441 003

BIG2 antibody - 441 003 K.D.

BIG2 is a guanine-nucleotide exchange factor
Rabbit polyclonal purified antibody
Cat. No.: 441 003
Amount: 50 µg
Price: $380.00
Cat. No. 441 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen Synthetic peptide corresponding to AA 1778 to 1791 from rat Big2 (UniProt Id: Q7TSU1)
Reactivity Reacts with: rat (Q7TSU1), mouse (A2A5R2).
Other species not tested yet.
Specificity Specific for BIG 2 K.D. validated PubMed: 15198677
Remarks

IHC: Detects high levels of endogenous BIG 2 protein (e.g. in hippocampus). Regions with low expression levels may be difficult to interpret.
Data sheet 441_003.pdf

References for BIG2 - 441 003

The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
Cat. No.: 441 003
Amount: 50 µg
Price: $380.00
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors.
Charych EI, Yu W, Miralles CP, Serwanski DR, Li X, Rubio M, De Blas AL
Journal of neurochemistry (2004) 901: 173-89. 441 003 WB, ICC, IHC, EM; KD verified; tested species: rat
ICC
ICC
IHC
Background

Brefeldin A-inhibited guanine exchange factor 2 (BIG 2) belongs to the guanine-nucleotide exchange factors (GEFs) for ADP-ribosylation factors (ARFs).

Together with its close relative BIG 1 it is involved in the regulation of membrane traffic through activating ARFs and the recruitment of coat protein complexes, such as the COPI and the AP-1 clathrin adaptor complex.

It has been shown to interact with the beta-subunits of the GABA type-A receptors.