|Cat. No. 441 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 (see remarks) gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 1778 to 1791 from rat Big2 (UniProt Id: Q7TSU1)|
Reacts with: rat (Q7TSU1), mouse (A2A5R2).
Other species not tested yet.
|Specificity||Specific for BIG 2 K.D. PubMed: 15198677|
IHC: Detects high levels of endogenous BIG 2 protein (e.g. in hippocampus). Regions with low expression levels may be difficult to interpret.
Brefeldin A-inhibited guanine exchange factor 2 (BIG 2) belongs to the guanine-nucleotide exchange factors (GEFs) for ADP-ribosylation factors (ARFs).
Together with its close relative BIG 1 it is involved in the regulation of membrane traffic through activating ARFs and the recruitment of coat protein complexes, such as the COPI and the AP-1 clathrin adaptor complex.
It has been shown to interact with the beta-subunits of the GABA type-A receptors.