|Cat. No. 438 004||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 1000 up to 1 : 2000 gallery
|Immunogen||Synthetic sulfated CCK-8 peptide corresponding to AA 96 to 103 from mouse CCK precursor (UniProt Id: P09240).|
Reacts with: mouse (P09240), rat (P01355), human (P06307).
Other species not tested yet.
|Specificity||The antibody recognizes CCK-8. It may crossreact with the precursor protein and with other peptides of the cholecystokinin family due to sequence homology.|
Cholecystokinin (CCK) is synthesized as a preprohormone, which is then converted into multiple isoforms. The sulphated octapeptide amide CCK-8 exists as the predominant form of CCK in neurons.
CCK is widely distributed in several brain regions, including hypothalamus, hippocampus, amygdala nuclei, and cortical regions. In the periphery, CCK peptides are mainly produced in small intestinal endocrine I-cells and in neurons of the enteric nervous system.
CCK plays important physiological roles both as a neuropeptide in the central nervous system and as a peptide hormone in the gut. It is released rapidly into the circulation in response to a meal. The greatest stimulator of CCK release is the presence of fatty acids and/or certain amino acids in the chyme entering the duodenum. CCK peptides stimulate pancreatic enzyme secretion and growth, gallbladder contraction, gut motility, and inhibit gastric acid secretion.
In the central nervous system, CCK acts as a neurotransmitter and neuromodulator regulating both the electrical activity of neurons and the release of other neuropeptides. It is involved in feeding, satiety, pain, anxiety, and memory processes.