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Home > Products > 436 004

Secretagogin antibody - 436 004

Secretagogin is an EF-hand calcium binding protein
Guinea pig polyclonal antiserum
Cat. No.: 436 004
Amount: 100 µl
Price: $365.00
Cat. No. 436 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 up to 1 : 2000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 276 from mouse Secretagogin (UniProt Id: Q91WD9)
Reactivity Reacts with: rat (Q6R556), mouse (Q91WD9).
Other species not tested yet.
Data sheet 436_004.pdf

References for Secretagogin - 436 004

An integrated single-cell RNA-seq atlas of the mouse hypothalamic paraventricular nucleus links transcriptomic and functional types.
Berkhout JB, Poormoghadam D, Yi C, Kalsbeek A, Meijer OC, Mahfouz A
Journal of neuroendocrinology (2024) 362: e13367. 436 004 IHC; tested species: mouse
Cat. No.: 436 004
Amount: 100 µl
Price: $365.00
An integrated single-cell RNA-seq atlas of the mouse hypothalamic paraventricular nucleus links transcriptomic and functional types.
Berkhout JB, Poormoghadam D, Yi C, Kalsbeek A, Meijer OC, Mahfouz A
Journal of neuroendocrinology (2024) 362: e13367. 436 004 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC
IHC
IHC-P
Background

Secretagogin, also known as SCGN or SECRET, is a member of the EF-hand (E-helix-loop-F-helix-hand) superfamily of calcium-binding proteins with homology to calbindin-D28k (CALB1) and calretinin (CALB2) which are widely expressed in cells and tissues. Calcium-binding proteins of different subfamilies regulate calcium as a second messenger. Calbindin, calretinin, calmodulin, S-100, parvalbumin and troponin C are members of the low molecular weight calcium-binding protein family.

Interaction between secretagogin and vesicle trafficking proteins, including SNAP25, SNAP23, ARFGAP2, and DOC2A, suggests a possible role for SCGN in neurotransmitter and hormone release.

The secretagogin protein was isolated from pancreatic islets of Langerhans and insulinomas, and shows abundant pancreatic expression and reduced expression in thyroid, adrenal medulla, stomach, small intestine, and colon.
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