Synaptic Systems - Aquaporin4

Cat. No.: 429 011BT

Amount: 100 µg

Price: $485.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

ICC
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IHC
(3 img)

IHC-P (FFPE)
(3 img)

Background

Aquaporin 4 (AQP4) is the most abundant bidirectional water-channel in the central nervous system. It is particularly abundant in astrocytes, where it is highly concentrated in the perivascular end-feet surrounding blood vessels and at the interfaces between brain tissue and cerebrospinal fluid (1). AQP4 is also expressed in ependymal cells and, outside the CNS, in tissues including the kidney, skeletal muscle, lung, stomach and several glandular epithelia (2,3).
It contributes to the regulation of water and ion homeostasis, extracellular volume, brain edema and blood–brain barrier function. AQP4 is also an important component of the glymphatic system, which facilitates the movement and clearance of substances through the brain interstitial space (1,4).
Alterations in AQP4 expression or localization have been implicated in a number of pathological conditions including neuroinflammation, brain edema, traumatic brain injury, neurodegenerative disorders and neuromyelitis optica spectrum disorders (NMOSD)(4).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Nagelhus EA et al., Physiol. Rev. (2013)
2 ) Palazzo C et al., Int J Mol Sci (2020)
3 ) Gleiser C et al., Int J Mol Sci (2016)
4 ) Wang Y et al., Aging Dis (2022)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 429 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P (FFPE): 1 : 1000 up to 1 : 2000 gallery Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	325B5A5
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity	Reacts with: mouse (P55088-1), rat (P47863), human (P55087). Other species not tested yet.
Matching control protein/peptide	429-0P
Data sheet	Data Sheet 429_011bt Safety Data Sheet (SDS)

Aquaporin4 antibody - 429 011BT