Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Aquaporin4 antibody - 429 011BT

Aquaporin4 is a water-specific channel in the blood brain barrier
Mouse monoclonal purified IgG
Cat. No.: 429 011BT
Amount: 100 µg
Price: $480.00
Cat. No. 429 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Label biotin
Clone 325B5A5
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity Reacts with: mouse (P55088-1), rat (P47863), human (P55087).
Other species not tested yet.
Matching control protein/peptide 429-0P
Data sheet 429_011bt.pdf
Cat. No.: 429 011BT
Amount: 100 µg
Price: $480.00
Background

Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.