Synaptic Systems - Aquaporin4

Cat. No.: 429 008

Amount: 50 µg

Price: $420.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

ICC
(2 img)

IHC
(3 img)

IHC-P (FFPE)
(5 img)

IHC-Fr
(3 img)

IHC-G
(2 img)

Background

Aquaporin 4 (AQP4) is the most abundant bidirectional water-channel in the central nervous system. It is particularly abundant in astrocytes, where it is highly concentrated in the perivascular end-feet surrounding blood vessels and at the interfaces between brain tissue and cerebrospinal fluid (1). AQP4 is also expressed in ependymal cells and, outside the CNS, in tissues including the kidney, skeletal muscle, lung, stomach and several glandular epithelia (2,3).
It contributes to the regulation of water and ion homeostasis, extracellular volume, brain edema and blood–brain barrier function. AQP4 is also an important component of the glymphatic system, which facilitates the movement and clearance of substances through the brain interstitial space (1,4).
Alterations in AQP4 expression or localization have been implicated in a number of pathological conditions including neuroinflammation, brain edema, traumatic brain injury, neurodegenerative disorders and neuromyelitis optica spectrum disorders (NMOSD)(4).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Nagelhus EA et al., Physiol. Rev. (2013)
2 ) Palazzo C et al., Int J Mol Sci (2020)
3 ) Gleiser C et al., Int J Mol Sci (2016)
4 ) Wang Y et al., Aging Dis (2022)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

IHC-Fr

IHC-G

IP

Standard Condition

Other products for this target

Cat. No. 429 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P (FFPE): 1 : 4000 gallery IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-G: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	Rb325B5A5
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity	Reacts with: mouse (P55088-1), rat (P47863), human (P55087), monkey. Other species not tested yet.
Matching control protein/peptide	429-0P
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 325B5A5. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells IHC: Antigen retrieval with formic acid is tolerated. IHC-Fr: The following fixatives are possible: methanol, 4% formaldehyde/PFA. IHC-G: 9% glyoxal fixation is recommended.
Data sheet	Data Sheet 429_008 Safety Data Sheet (SDS)

Aquaporin4 antibody - 429 008