Cat. No.: 429 008
Amount: 50 µg
Price:
$415.00
Cat. No. 429 008 |
50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 4000 gallery Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.', $event)" style="cursor: help;">IHC-G: 1 : 500 up to 1 : 1000 (see remarks) gallery |
Clone | Rb325B5A5 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 249 to 323 from mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1) |
Reactivity |
Reacts with: mouse (P55088-1), rat (P47863), human (P55087). Other species not tested yet. |
Matching control protein/peptide | 429-0P |
Remarks |
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 325B5A5. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells |
Data sheet | 429_008.pdf |
Aquaporin4, or AQP4, also known as Mercurial-insensitive water channel (MIWC), is a member of the aquaporin water channel family. This osmoreceptor regulates body water balance and mediates water flow within the central nervous system.