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Home > Products > 428 003

FTO antibody - 428 003 K.D.

A Fat mass and obesity-associated protein
Rabbit polyclonal purified antibody
Cat. No.: 428 003
Amount: 50 µg
Price: $375.00
Cat. No. 428 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 2000 up to 1 : 10000 AP-staining gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 2 to 67 from mouse FTO (UniProt Id: Q8BGW1)
Reactivity Reacts with: mouse (Q8BGW1), rat (Q2A121).
Other species not tested yet.
Predicted to cross-react with human (Q9C0B1) due to high sequence homology.
Specificity In human it detects exclusively isoform 1. Isoforms 2, 3 and 4 lack the N-terminus that contains the antibody epitope. In mouse and rat these isoforms lacking the N-terminus have not been described. K.D. validated
Data sheet 428_003.pdf
Cat. No.: 428 003
Amount: 50 µg
Price: $375.00
WB
ICC
ICC
IHC-P
Background

Fat mass and obesity-associated protein also known as alpha-ketoglutarate-dependent dioxygenase FTO is an RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs. It has been shown to act as a regulator of fat mass, adipogenesis and energy homeostasis.  FTO specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.