FTO antibody - 428 003 K.D.

A Fat mass and obesity-associated protein

Rabbit polyclonal purified antibody

Cat. No.: 428 003

Amount: 50 µg

Price: $380.00

Cat. No. 428 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 2000 up to 1 : 10000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 67 from mouse FTO (UniProt Id: Q8BGW1)
Reactivity	Reacts with: mouse (Q8BGW1), rat (Q2A121). Other species not tested yet. Predicted to cross-react with human (Q9C0B1) due to high sequence homology.
Specificity	In human it detects exclusively isoform 1. Isoforms 2, 3 and 4 lack the N-terminus that contains the antibody epitope. In mouse and rat these isoforms lacking the N-terminus have not been described. K.D. validated
Data sheet	Datasheet 428_003

Cat. No.: 428 003

Amount: 50 µg

Price: $380.00

ICC

IHC-P

Background

Fat mass and obesity-associated protein also known as alpha-ketoglutarate-dependent dioxygenase FTO is an RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs. It has been shown to act as a regulator of fat mass, adipogenesis and energy homeostasis. FTO specifically demethylates N₆-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.

1 ) Jia G et al., Nat. Chem. Biol. (2011)
2 ) Zhou J et al., Nature (2015)
3 ) Wei J et al., Mol. Cell (2018)

Protocols

ICC

Fixed Cells

IHC-P