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FTO - 428 003

A Fat mass and obesity-associated protein
Polyclonal rabbit purified antibody
Cat. No.: 428 003
Amount: 50 µg
Price: $370.00
Cat. No. 428 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 2000 up to 1 : 10000 AP-staining gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: 1 : 1000 gallery  
Immunogen Recombinant protein corresponding to AA 2 to 67 from mouse FTO (UniProt Id: Q8BGW1)
Reactivity Reacts with: mouse (Q8BGW1), human (Q9C0B1).
Other species not tested yet.
Specificity In human it detects exclusively isoform 1. Isoforms 2, 3 and 4 lack the N-terminus that contains the antibody epitope. In mouse and rat these isoforms lacking the N-terminus have not been described. K.D.
Data sheet 428_003.pdf
Cat. No.: 428 003
Quantity: 50 µg
Price: $370.00
Background

Fat mass and obesity-associated protein also known as alpha-ketoglutarate-dependent dioxygenase FTO is an RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs. It has been shown to act as a regulator of fat mass, adipogenesis and energy homeostasis.  FTO specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.