Synaptic Systems

Cat. No.: 421 002

Amount: 200 µl

Price: $355.00

Cat. No. 421 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 19 to 917 from mouse MDGA1 (UniProt Id: Q0PMG2)
Reactivity	Reacts with: mouse (Q0PMG2). Other species not tested yet.
Specificity	K.O. validated PubMed: 35532105
Data sheet	421_002.pdf

References for MDGA1 - 421 002

Functional Neuroligin-2-MDGA1 interactions differentially regulate synaptic GABAARs and cytosolic gephyrin aggregation.
Zeppillo T, Ali H, Ravichandran S, Ritter TC, Wenger S, López-Murcia FJ, Gideons E, Signorelli J, Schmeisser MJ, Wiltfang J, Rhee J, et al.
Communications biology (2024) 71: 1157. 421 002 WB, IHC-Fr; KO verified; tested species: mouse

MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior.
Toledo A, Letellier M, Bimbi G, Tessier B, Daburon S, Favereaux A, Chamma I, Vennekens K, Vanderlinden J, Sainlos M, de Wit J, et al.
eLife (2022) 11: . 421 002 WB, IP, IHC; KO verified; tested species: mouse

Proteomics analysis of the brain from a Gaucher disease mouse identifies pathological pathways including a possible role for transglutaminase 1.
Blumenreich S, Ben-Yashar DP, Shalit T, Kupervaser M, Milenkovic I, Joseph T, Futerman AH
Journal of neurochemistry (2024) 1681: 52-65. 421 002 WB, IHC; KD verified; tested species: rat

Cat. No.: 421 002

Amount: 200 µl

Price: $355.00

IHC

Background

MAM domain GPI anchor 1 and 2 proteins (MDGA1 and MDGA2) are Ig superfamily adhesion molecules composed of six IG domains, a fibronectin III domain, a MAM domain, and a GPI anchor.

MDGAs are required for radial migration and positioning of cortical neurons making it a useful layer and area specific marker during neural development.

They play a role in the formation or maintenance of Neuroligin2 positive inhibitory synapses.

1 ) Lee K et al., Proc. Natl. Acad. Sci. U.S.A. (2013)
2 ) Takeuchi A et al., J. Neurosci. (2006)
3 ) Fujimura Y et al., Brain Res. (2006)
4 ) Ishikawa T et al., Dev. Dyn. (2011)

Protocols

IHC

MDGA1 antibody - 421 002 K.O.

References for MDGA1 - 421 002