Synaptic Systems - MAP1B-LC1

Cat. No.: 410 005

Amount: 50 µg

Price: $465.00

WB

Background

MAP1B, also referred to as Microtubule-associated protein 1B, MAP5, MAP1.2, or MAP1X, belongs to the microtubule-associated protein family.
The MAP1B gene encodes for a single precursor protein that is subsequently cleaved into a heavy chain of 280 kDa (MAP1B-HC) and a light chain of 32 kDa (MAP1B-LC1). Both chains have a microtubule-binding domain and an actin-binding domain, and they associate to form a multimeric complex.
MAP1B is mainly expressed in neurons. It is present at its highest levels in the brain during early postnatal development. In the adult, MAP1B expression remains elevated in brain regions that retain a high level of axonal growth and synaptic plasticity.
MAP1B is involved in microtubule and actin filament association. It is required for proper axon growth and dendritic spine morphogenesis in developing neurons.
In the last few years, it has become apparent that MAP1B has other cellular and molecular functions that are not related to its microtubule-stabilizing properties.
In line with this, MAP1B-LC1 has additional functions outside of the complex with the heavy chain. It associates with various proteins like serotonin receptor 5-HT6R, α1-syntrophin, GRIP1, Tiam1, and p53. Accordingly, MAP1B-LC1 may be considered a signaling protein that regulates molecular pathways.

1 ) Palenzuela R et al., J. Neurosci. (2017)
2 ) Villarroel-Campos D et al., Dev Neurobiol (2014)
3 ) Kim SH et al., PLoS ONE (2014)
4 ) Henríquez DR et al., PLoS ONE (2012)
5 ) Fuhrmann-Stroissnigg H et al., PLoS ONE (2012)
6 ) Lee SY et al., FEBS Lett. (2008)

Protocols

Cat. No. 410 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery
Immunogen	Synthetic peptide corresponding to AA 2383 to 2396 from mouse MAP1B (UniProt Id: P14873)
Reactivity	Reacts with: mouse (P14873). Weaker signal: rat (P15205). Other species not tested yet.
Data sheet	410_005.pdf

MAP1B-LC1 antibody - 410 005