|Cat. No. 410 005||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
|Applications||WB: 1 : 1000 (AP staining) gallery|
|Immunogen||Synthetic peptide corresponding to AA 2383 to 2396 from mouse MAP1B (UniProt Id: P14873)|
Reacts with: mouse (P14873).
Weaker signal: rat (P15205).
Other species not tested yet.
|Specificity||Specific for MAP1B-LC1|
MAP1B, also referred to as Microtubule-associated protein 1B, MAP5, MAP1.2, or MAP1X, belongs to the microtubule-associated protein family.
The MAP1B gene encodes for a single precursor protein that is subsequently cleaved into a heavy chain of 280 kDa (MAP1B-HC) and a light chain of 32 kDa (MAP1B-LC1). Both chains have a microtubule-binding domain and an actin-binding domain, and they associate to form a multimeric complex.
MAP1B is mainly expressed in neurons. It is present at its highest levels in the brain during early postnatal development. In the adult, MAP1B expression remains elevated in brain regions that retain a high level of axonal growth and synaptic plasticity.
MAP1B is involved in microtubule and actin filament association. It is required for proper axon growth and dendritic spine morphogenesis in developing neurons.
In the last few years, it has become apparent that MAP1B has other cellular and molecular functions that are not related to its microtubule-stabilizing properties.
In line with this, MAP1B-LC1 has additional functions outside of the complex with the heavy chain. It associates with various proteins like serotonin receptor 5-HT6R, α1-syntrophin, GRIP1, Tiam1, and p53. Accordingly, MAP1B-LC1 may be considered a signaling protein that regulates molecular pathways.