RFP antibody - 409 009

RFP is a red fluorescent protein tag

Chicken monoclonal recombinant IgY

Cat. No.: 409 009

Amount: 50 µg

Price: $415.00

Cat. No. 409 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch337B1
Subtype	IgY (λ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 219 from sea anemone mScarlet
Specificity	Recognizes mRFP, mCherry, mOrgange2, tdTomato, mScarlet. Does not recognize dsRed1 and 2, mKate2, mRuby3 and Tag RFP.
Remarks	The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 337B1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	409_009.pdf

Cat. No.: 409 009

Amount: 50 µg

Price: $415.00

ICC

IHC

Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D et al., Genesis (2010)
2 ) Shaner NC et al., Nat. Biotechnol. (2004)
3 ) Labas YA et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
4 ) Fradkov AF et al., FEBS Lett. (2000)

Protocols

ICC

IHC

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