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RFP antibody - 409 009

RFP is a red fluorescent protein tag
Chicken monoclonal recombinant IgY
Cat. No.: 409 009
Amount: 50 µg
Price: $415.00
Cat. No. 409 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not recommended
Clone Ch337B1
Subtype IgY (λ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 219 from sea anemone mScarlet
Specificity Recognizes mRFP, mCherry, mOrgange2, tdTomato, mScarlet. Does not recognize dsRed1 and 2, mKate2, mRuby3 and Tag RFP.

The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 337B1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 409_009.pdf
Cat. No.: 409 009
Amount: 50 µg
Price: $415.00
Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.