Synaptic Systems

Cat. No.: 400 203

Amount: 50 µg

Price: $375.00

Cat. No. 400 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 189 to 283 from rat TMEM119 (UniProt Id: B2RYL3)
Reactivity	Reacts with: rat (B2RYL3). Weaker signal: mouse (Q8R138). Other species not tested yet.
Data sheet	400_203.pdf

References for TMEM119 - 400 203

Significant Sex Differences in the Efficacy of the CSF1R Inhibitor-PLX5622 on Rat Brain Microglia Elimination.
Sharon A, Erez H, Spira ME
Pharmaceuticals (Basel, Switzerland) (2022) 155: . 400 203 IHC; tested species: rat

Inflammatory Foreign Body Response Induced by Neuro-Implants in Rat Cortices Depleted of Resident Microglia by a CSF1R Inhibitor and Its Implications.
Sharon A, Jankowski MM, Shmoel N, Erez H, Spira ME
Frontiers in neuroscience (2021) 15: 646914. 400 203 IHC; tested species: rat

Immunohistological and Ultrastructural Study of the Inflammatory Response to Perforated Polyimide Cortical Implants: Mechanisms Underlying Deterioration of Electrophysiological Recording Quality.
Huang SH, Shmoel N, Jankowski MM, Erez H, Sharon A, Abu-Salah W, Nelken I, Weiss A, Spira ME
Frontiers in neuroscience (2020) 14: 926. 400 203 IHC; tested species: rat

Cat. No.: 400 203

Amount: 50 µg

Price: $375.00

IHC

Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.

1 ) Bennett ML et al., Proc. Natl. Acad. Sci. U.S.A. (2016)
2 ) Satoh J et al., Neuropathology (2016)

Protocols

IHC

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TMEM119 antibody - 400 203

References for TMEM119 - 400 203