TMEM119 antibody mouse specific - 400 009

TMEM119 is a microglia specific transmembrane protein

Chicken monoclonal recombinant IgY

Cat. No.: 400 009

Amount: 50 µg

Price: $415.00

Cat. No. 400 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: 1 : 250 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch195H4
Subtype	IgY (λ light chain)
Immunogen	Recombinant protein corresponding to the C-terminal region of mouse TMEM119 (UniProt Id: Q8R138)
Reactivity	Reacts with: mouse (Q8R138). No signal: rat (B2RYL3), human (Q4V9L6). Other species not tested yet.
Remarks	This antibody is recommended for mouse only. Due to significant differences of TMEM 119 among species, cross-reactivity is unlikely. The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 195H4. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	400_009.pdf

Cat. No.: 400 009

Amount: 50 µg

Price: $415.00

IHC

IHC-P

Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.

1 ) Bennett ML et al., Proc. Natl. Acad. Sci. U.S.A. (2016)
2 ) Satoh J et al., Neuropathology (2016)

Protocols

IHC

IHC-P

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